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牛津遗传学/pSF-TEF1-SnapFusion（OG604）酵母SnapFusion克隆质粒/OG604/1ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-TEF1-SnapFusion
ProductCode:OG604
Size(bp):6729bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:YeastElongationFactorAlpha-1(TEF-1)promoter
PlasmidPurpose:
ThisvectorisastandardSaccharomycescerevisiaeyeastexpressionplasmidwiththeexceptionthatithasbeenmodifiedtoallowthedirectcloningofgenefragmentsthathavebeendesignedtofitwithanyofourpeptidetagplasmidrange.Thiscanbeusefulifyouhavecreatedapeptidefusionbutyouwouldalsoliketocreateanon-taggedgenevariant.ThemultiplecloningsitecontainstwoBsgIandBseRIsitesthatproduceoverhangsthatarecompatIBLewithgenedesignedusingourSnapFusionprimerdesigner(seethecloningtabforthisproduct).Thepromoterinthisplasmid(TEF1)isastrongconstitutiveyeastpromoter.
PromoterExpressionLevel:Thisplasmidcontainstheyeasttranslationelongationfactor1promoter.ItisthestrongestpromoterthatweprovideforexpressioninSaccharomycescerevisiae.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsforyeastbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
