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Oxford Genetics/pSF-T7-EMCV (OG185) T7 IRES Expression Plasmid/OG185/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-T7-EMCV
ProductCode:OG185
Size(bp):4378bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:T7PolymerasePromoter
PlasmidPurpose:
Thisvectorallowstranscriptionofun-polyadenylatedandun-cappedRNAusingtheT7bacteriophagepolymerase.Thisplasmidalsocontainstheinternalribosomeentrysite(IRES)fromEncephalomyocarditisvirus(ECMV)betweenthepromoterandthemultiplecloningsite(MCS).ItisdesignedtobeusedtoexpressgenesusingeukaryoticT7expressionsystemssuchasthoseusedfortherecoveryofavarietyofviruses.ThisvectorisnotdesignedfortheexpressionoftwoproteinsfromthesamemRNA.ForthispurposewehavedesignedpSF-CMV-MCS-IRES-PciIwherethePciIsitecontainsastartcodoninthecorrectpositionfortheIREStoexpressefficiently.InthisvectorthereisanNcoIsite(whichcontainsanATGstartcodon)inthecorrectpositiontoenableefficientexpressionofageneintheMCS.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
