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Oxford Genetics/pSF-Renilla-Photinus (OG595) Bi-directional Firefly Renilla Luciferase Plasmid/OG595/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-Renilla-Photinus
ProductCode:OG595
Size(bp):6170bp
BacterialAntibioticSelection:AmpR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:
PlasmidPurpose:
Thisplasmidhasbeendesignedsothatsequencescaninsertedbetweentworeportergenes(renillaandfireflyluciferase)toallowtherelativetranscriptionlevelinbothdirectionstobedeterminedbymeasuringthereporterluminescence.DualluciferasereporterassaykitsareavailablefromotherprovidersthatarecompatIBLewiththisplasmid.TheplasmidonlycontainsaBgl2sitebetweenthetwogenestoallowinsertion.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
