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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Oxford Genetics/pSF-PGK-Cas9WT (OG3541) CRISPR/Cas9 Expression Plasmid/OG3541/1 Ea
商品详细Oxford Genetics/pSF-PGK-Cas9WT (OG3541) CRISPR/Cas9 Expression Plasmid/OG3541/1 Ea
Oxford Genetics/pSF-PGK-Cas9WT (OG3541) CRISPR/Cas9 Expression Plasmid/OG3541/1 Ea
Oxford Genetics/pSF-PGK-Cas9WT (OG3541) CRISPR/Cas9 Expression Plasmid/OG3541/1 Ea
商品编号: OG3541
品牌: oxgene
市场价: ¥7047.40
美元价: 4228.44
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PlasmidInfo:

PlasmidInformation

ProductName:pSF-PGK-Cas9WT

ProductCode:OG3541

Size(bp):8277bp

BacterialAntibioticSelection:KanR

OriginandCompatibility:pUChighcopyderivedfrompBR322

BacterialCopyNumber:500-700percell

Promoter:MousePhosphoglycerateKinase(PGK)Promoter

PlasmidPurpose:

ThisplasmidisdesignedtoexpresswildtypeCas9inmammaliancells.ExpressionisdrivenfromaPGKpromoterinthemainMCS.

PromoterExpressionLevel:

Themurinephosphoglyceratekinase(PGK)promoterdrivingexpressionallowsforlongtermstableexpressioninvitroandinvivoinmosttypes. 

SequenceandMap:

OtherInfo:

TranscriptionTermination:

Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.

Cloning:

Cloninginagene:

Thisplasmidcontainsagenewithinthemainmultiplecloningsite(NotI-ClaI).Anyplasmidthatwesellwherethegeneisthisconfigurationwillbelocatedintheexactsamepositioninrelationtothestartandstopcodonofthegeneintheplasmid.TheonlyexceptionstothisrulearefusionsproteinswherethefusiongenemaybepositionedatthefrontorendoftheMCStoallowgenefusion.

Bypositioningallofourgenesinthesamelocationitallowsthemtobetransferredbetweenplasmidsusingthesamecloningmethodandrestrictionsitesregardlessoftheplasmidbeingusedfromourproductrange.InsertinganewgeneintothisplasmidshouldbeeasilypossIBLeusingarangeofstandardrestrictionenzymesitesthatflankthegenecurrentlyinthevector.

Multiplecloningsitenotes:

InthemultiplecloningsitetherearetwoimportantrestrictionsitescalledBsgIandBseRIsites.ThesesitesbothcuttheDNAatthesamepositionandcleavethestopcodonofthegeneinthemultiplecloningsiteinthisplasmidtherebyproducingaTAoverhang.Thisoverhangiscompatiblewithanyofourpeptideorreporterfusiontagplasmidsalsocutwitheitheroftheseenzymes.ThisallowsseamlessC-terminalfusionstobemadewiththegeneinthismultiplecloningsiteusingasinglecloningstepfromourC-terminalpeptideandreportertagproductrange.NormallytheeasiestmethodistoclonetheC-terminaltagfromourotherplasmidproductsintothisplasmidusingBsgI or BseRIandthedownstreamClaIrestrictionsite.

BseRIandBsgIsitesarenon-palindromicandcleaveadefinednumberofbasesawayfromtheirbindingsites.Thisallowsthemtocuttheupstreamstopcodoninthegeneinthisplasmidregardlessofthegenesequence.

IPStatus:

IntellectualPropertyStatus

ThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere

品牌介绍
OXGENE的技术平台和专家解决方案加速了细胞和基因疗法的发现,开发和制造。我们解决了现代生物学中基因治疗,抗体治疗和基因编辑方面最重要和最具挑战性的问题。我们的技术可以实现精确而强大的哺乳动物细胞工程。我们以自动化和信息学为导向的方法意味着我们解决了其他人无法推进新疗法交付的问题。