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Oxford Genetics/pSF-pA-MinProm-SEAP (OG248) Minimal Promoter SEAP Plasmid/OG248/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-pA-MinProm-SEAP
ProductCode:OG248
Size(bp):5572bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:HSVTKMinimalPromoter
PlasmidPurpose:
Thisplasmidcontainsamultiplecloningsitethatisupstreamofaminimalpromoterthatisupstreamofthehumansecretedalkalinephosphatase(SEAP)reportergene.ThisplasmidcanbeusedtoinsertspecificDNAsequencesthatwillendowtheminimalpromoterwithspecificbiochemicalpropertiessuchastissuespecificityortranscriptionfactorspecificactivation.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
Cloning:
MultipleCloningSiteNotes:ThereisastartcodonintheNcoIsiteintheMCSthatcanberemovedbydigestionwithKpnIifrequired.TheMCSforgeneinsertionsextendsfromNotItoXbaIbuttherearebuiltinShine-DalgarnoandKozakribosomalentrysitesequencesalignedwiththestartcodonintheNcoIsite.TheClaIandNheIsiteshaveotherfunctionsandallowtheinsertionofsequencesfromourothercatalogueplasmids(suchasIRESsequencesandsecondpromoters).
TheBsgIandBseRIrestrictionsitesintheplamsidcleavewithinthestopcodonintheXbaIsiteintheMCS.Thiscanallowretrospectivefusionofcodingsequencestoourpeptidetagrangeifyourgenesstopcodonispositionedhere.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
