PlasmidInfo:
PlasmidInformation
ProductName:pSF-pA-MinProm-Fluc
ProductCode:OG246
Size(bp):5659bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:HSVTKMinimalPromoter
PlasmidPurpose:
ThisplasmidcontainsamultiplecloningsitethatisupstreamofaminimalpromoterthatisupstreamofthePhotinuspyralis(FLuc)luciferase.ThisplasmidcanbeusedtoinsertspecificDNAsequencesthatwillendowtheminimalpromoterwithspecificbiochemicalpropertiessuchastissuespecificityortranscriptionfactorspecificactivation.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
Cloning:
MultipleCloningSiteNotes:ThereisastartcodonintheNcoIsiteintheMCSthatcanberemovedbydigestionwithKpnIifrequired.TheMCSforgeneinsertionsextendsfromNotItoXbaIbuttherearebuiltinShine-DalgarnoandKozakribosomalentrysitesequencesalignedwiththestartcodonintheNcoIsite.TheClaIandNheIsiteshaveotherfunctionsandallowtheinsertionofsequencesfromourothercatalogueplasmids(suchasIRESsequencesandsecondpromoters).
TheBsgIandBseRIrestrictionsitesintheplamsidcleavewithinthestopcodonintheXbaIsiteintheMCS.Thiscanallowretrospectivefusionofcodingsequencestoourpeptidetagrangeifyourgenesstopcodonispositionedhere.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
