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Oxford Genetics/pSF-OXB20-SnapFusion (OG605) Bacterial SnapFusion Constiutive Plasmid/OG605/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-OXB20-SnapFusion
ProductCode:OG605
Size(bp):3866bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:OXB20strongconstitutivebacterialpromoter
PlasmidPurpose:
ThisvectorisastandardE.coliexpressionplasmidwiththeexceptionthatithasbeenmodifiedtoallowthedirectcloningofgenefragmentsthathavebeendesignedtofitwithanyofourpeptidetagplasmidrange.Thiscanbeusefulifyouhavecreatedapeptidefusionbutyouwouldalsoliketocreateanon-taggedgenevariant.ThemultiplecloningsitecontainstwoBsgIandBseRIsitesthatproduceoverhangsthatarecompatIBLewithgenedesignedusingourSnapFusionprimerdesigner(seethecloningtabforthisproduct).Thepromoterinthisplasmidisastrongconstitutivebacterialpromoterthatdoesnotrequireinductionforactivity.
PromoterExpressionLevel:ThisplasmidcontainsaverystrongconstitutiveE.colipromoterthatwasderivedfromtheRecApromoterbyremovingtheLexArepressorsite.Itispartofourconstitutivebacterialpromoterrange.ThispromotershowsthehighestlevelofexpressioninthepromoterrangewithOXB1showingthelowestlevel.Theyrequirenoinducingagentforexpression.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
