PlasmidInfo:
PlasmidInformation
ProductName:pSF-Nano-Kan
ProductCode:OG3590
Size(bp):2194bp
BacterialAntibioticSelection:AmpR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:
PlasmidPurpose:
ThisvectorwasdesignedtobeassmallaspossIBLewhilestillpossessingallofthenecessarycomponentstoreplicateinbacterialcells.ItcontainsthekanamycinresistanceMarker.
PromoterExpressionLevel:Thispromoterdoesnotcontainapromoter,however,itcontainsrestrictionsitesthatallowanyofthepromotersfromourotherproductstotransferredintothisplasmidusingstandardcloningtechniques.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmiddoesnotcontainaterminatorofanykindotherthantheterminatorforthebacterialresistancegene.
Cloning:
Cloninginagene:Thisplasmidhasbeendesignedtobecompatiblewitharangeofcloningtechniques.Themultiplecloningsitecontainsarangeofstandardcommonlyusedrestrictionsitesforcloning.UsingthesesitesgenescanbeinsertedusingstandardcloningmethodswithDNAligase.Othermethodssuchasligaseindependentcloning(LIC)GibsonAssemblyInFusionHDorSeamlessGeneArtcanalsobeusedandbecauseallofourplasmidsarebasedonthesamebackbonethesamemethodcanbeusedforcloningintoallofourcataloguevectors.
Multiplecloningsitenotes:
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
