Plasmid Info:

Plasmid Purpose:

This cloning plasmid is designed for the production and expression of rat (Rattus rattus) lambda light chain antibody genes. The constant region of the antibody lambda gene has been positioned adjacent to a BseRI restriction site in this plasmid which when cleaved produces an overhang within the first codon of the antibody gene. This allows variable regions of antibodies to be cloned in creating seamless fusion between the the variable and constant regions. As part of this product range we can also provide expression plasmids for both human and mouse antibodies as well as cloning vectors for rat IgG heavy chain and Kappa light chain (pSF-CMV-Rat-IgG HC and pSF-CMV-Rat-Kappa respectively).

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

Sequence and Map:

Other Info:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

IP Status:

This product is part of our SnapFast Pro plasmid range, for more information on the Intellectual property status of this plasmid please click here