PlasmidInfo:
PlasmidInformation
ProductName:pSF-CMV-Rat-Kappa_LC
ProductCode:OG525
Size(bp):4576bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:Cytomegalovirus(CMV)immediateearlypromoter
PlasmidPurpose:
Thisvectorisfortheproductionofkappaantibodylightchainsforrat(Rattusrattus).IthasbeenengineeredsothatwhenthevectoriscutwiththerestrictionenzymeBseRIitproducesanoverhangfromthefirstcodonoftheconstantregion.Thisallowsantibodylightchainvariableregionstobeseamlesslyligatedintothisvectortocreatefulllengthratkappalightchainantibodyexpressioncassettes.AspartofthisvectorrangewealsoprovideplasmidsfortheproductionofbothhumanandmouseantibodiesaswellasvectorsforthecreationofHeavyIgGchainandLamBDalightchainratantibodies(namedpSF-CMV-Rat-IgGHCandpSF-CMV-Rat-Lambdarespectively)
PromoterExpressionLevel:ThisplasmidcontainsthemammalianCMVpromotertodrivegeneexpression.WehavetestedallofourmammalianpromotersinarangeofcelltypesandCMVisconsistentlythestrongestinthosewehavestudied.HowevertherearemanyreportsoftheCMVpromoterdemonstratingsilencingbymethylationinlong-termculture.ForthisreasonwestockarangeofotherpromotersthatarecompatIBLewiththisplasmidandareavailableonrequest.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
