PlasmidInfo:
PlasmidInformation
ProductName:pSF-CMV-Rat-IgG1_HC
ProductCode:OG524
Size(bp):5236bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:Cytomegalovirus(CMV)immediateearlypromoter
PlasmidPurpose:
Thisplasmidisdesignedforthegenerationandexpressionofheavychainrat(Rattusrattus)immunoglobulinantibodygenes.Thevectorhasbeenformattedsothatitcanbecleavedtoproducearestrictionsiteoverhang(usingBseRI)withinthefirstcodonoftheantibodyconstantregion.Thisallowsthevariableregionsofantibodiestobeinsertedtocreatefulllengthheavychainexpressioncassettes.AspartofthisproductrangewealsohaveantibodyvectorsfortheproductionofmouseandhumanantibodiesaswellasplasmidsfortheproductionofratKappaandLamBDaantibodylightchains(pSF-CMV-Rat-KappaandpSF-CMV-Rat-Lambdarespectively).
PromoterExpressionLevel:ThisplasmidcontainsthemammalianCMVpromotertodrivegeneexpression.WehavetestedallofourmammalianpromotersinarangeofcelltypesandCMVisconsistentlythestrongestinthosewehavestudied.HowevertherearemanyreportsoftheCMVpromoterdemonstratingsilencingbymethylationinlong-termculture.ForthisreasonwestockarangeofotherpromotersthatarecompatIBLewiththisplasmidandareavailableonrequest.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
