PlasmidInfo:
PlasmidInformation
ProductName:pSF-CMV-HuIgG1_HC
ProductCode:OG527
Size(bp):5248bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:Cytomegalovirus(CMV)immediateearlypromoter
PlasmidPurpose:
ThisexpressionplasmidcontainsthehumanIgGheavychainconstantregionandhasbeendesignedtoallowtheseamlessfusionofvariableantibodyfragmentstocreatefulllengthheavychainexpressionconstructs.ThevectorhasbeenengineeredtocontainaBseRIrestrictionenzymesiteupstreamoftheconstantdomainthatwhencleavedproducesanoverhangofthefirsttwobasesofthefirstcodonoftheconstantregion.Thisallowsvariableantibodyfragmentstobeclonedupstreamtocreatefulllengthantibodygenes.WealsoprovideplasmidsforhumanKappaandLamBDalightchainexpression(termedpSF-CMV-HuKappaLCandpSF-CMV-HuLambdaLCrepectively).WealsohavearangeofantibodyexpressionplasmidsformouseandratIgGheavyandlightchains.
PromoterExpressionLevel:ThisplasmidcontainsthemammalianCMVpromotertodrivegeneexpression.WehavetestedallofourmammalianpromotersinarangeofcelltypesandCMVisconsistentlythestrongestinthosewehavestudied.HowevertherearemanyreportsoftheCMVpromoterdemonstratingsilencingbymethylationinlong-termculture.ForthisreasonwestockarangeofotherpromotersthatarecompatIBLewiththisplasmidandareavailableonrequest.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
