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Oxford Genetics/pSF-CMV-COOH-EKT-GST2 (OG139) C-Terminal GST Plasmid/OG139/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-CMV-COOH-EKT-GST2
ProductCode:OG139
Size(bp):4899bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:Cytomegalovirus(CMV)immediateearlypromoter
PlasmidPurpose:
ThisvectoraddsaGSTtagpositionedatthe3primeend(C-terminus)ofagene(expressedprotein)thatiswithintheprimaryMCS(NotItoXbaI).Thetagcanbeusedtopurifyaproteinbybindingtoglutathioneandthetagcanalsobeusedtoincreasethesolubilityofrecombinantproteinproducedinbacterialcells.ForreferencethecodingsequenceoftheGSTtagisonebasepairoutofframewiththeTAGstopcodonthatisfoundwithintheupstreamXbaIsite.Thereisanenterokinasecleavagesiteimmediatelyupstreamofthistag(DDDDK)thatcanbeusedtoremovethetagfromapurifiedproteinifrequired.
PromoterExpressionLevel:ThisplasmidcontainsthemammalianCMVpromotertodrivegeneexpression.WehavetestedallofourmammalianpromotersinarangeofcelltypesandCMVisconsistentlythestrongestinthosewehavestudied.HowevertherearemanyreportsoftheCMVpromoterdemonstratingsilencingbymethylationinlong-termculture.ForthisreasonwestockarangeofotherpromotersthatarecompatIBLewiththisplasmidandareavailableonrequest.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
Cloning:
CloninginaGene:ThereisastartcodonintheNcoIsitecanberemovedbydigestionwithKpnIifrequired.TheMCSforgeneinsertionsextendsfromNotItoXbaI.TherearebuiltinShine-DalgarnoandKOZAKsequencesalignedwiththestartcodonwithintheNcoIrestrictionsite.Iftheseareremovedyouwillneedtoaddaribosomalentrysitetothe5primeendofyourgeneforoptimalexpression.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
