>
产品中心 >
Specialty_ELISA_kits >
Oxford Genetics/pSF-CMV-COOH-EKT-CMyc2 (OG57) C-Terminal CMyc Tag Plasmid/OG57/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-CMV-COOH-EKT-CMyc2
ProductCode:OG57
Size(bp):4272bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:Cytomegalovirus(CMV)immediateearlypromoter
PlasmidPurpose:
ThisvectoraddsaCMyctagthatallowsproteindetectionorpurificationusingantibodiesagainstthepeptide.TheCMyctagwillbepositionedatthe3primeend(Cterminus)ofagene(expressedprotein)intheprimaryMCS(NotItoXbaI).TheCMyctagcodingsequenceisEQKLISEEDL.Thereisanenterokinasecleavagesiteimmediatelyupstreamofthistag(DDDDK)thatcanbeusedtoremovethetagfromapurifiedprotein.
PromoterExpressionLevel:ThisplasmidcontainsthemammalianCMVpromotertodrivegeneexpression.WehavetestedallofourmammalianpromotersinarangeofcelltypesandCMVisconsistentlythestrongestinthosewehavestudied.HowevertherearemanyreportsoftheCMVpromoterdemonstratingsilencingbymethylationinlong-termculture.ForthisreasonwestockarangeofotherpromotersthatarecompatIBLewiththisplasmidandareavailableonrequest.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
Cloning:
CloninginaGene:ThereisastartcodonintheNcoIsitecanberemovedbydigestionwithKpnIifrequired.TheMCSforgeneinsertionsextendsfromNotItoXbaI.TherearebuiltinShine-DalgarnoandKOZAKsequencesalignedwiththestartcodonwithintheNcoIrestrictionsite.Iftheseareremovedyouwillneedtoaddaribosomalentrysitetothe5primeendofyourgeneforoptimalexpression.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
