>
产品中心 >
Specialty_ELISA_kits >
Oxford Genetics/pSF-CAG-MCS-pA-CAG-MCS-pA (OG4116) Dual Promoter CAG with PolyA Signal Expression Plasmid/OG4116/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pSF-CAG-MCS-pA-CAG-MCS-pA
ProductCode:OG4116
Size(bp):7362bp
BacterialAntibioticSelection:KanR
OriginandCompatibility:pUChighcopyderivedfrompBR322
BacterialCopyNumber:500-700percell
Promoter:CAGsyntheticmammalianpromoterandUbiquitinpromoter/CAGsyntheticmammalianpromoterandUbiquitinpromoter/ChickenBetaActin(CBA)promoter/ChickenBetaActin(CBA)promoter
PlasmidPurpose:
Thismammalianexpressionvectorcontainstwo chimericCAGpromoterexpressioncassettewithtwouniqueMCSregionforcloningyourgeneofinterest.TheCAGpromoterisacompositeoftheCMVenhancer,thechickenbetaactinpromoter(CBA)andtherabbitbetaglobinintron.ThispromoterisoftenusedinsteadoftheCMVpromoterbecauseinsomecelltypestheCMVpromotercangetsilenced.TopreventthistheCAGpromotercontainstheCpGislandfromtheCBApromotertohelppreventpromotermethylation.Theplasmidcanbeusedtodrive expressionofuptotwoproteinsinarangeofmammaliancelltypesandwehaveconsistentlyfoundthatexpressionlevelsareequaltoCMVinshorttermexperimentsinstandardcelltypes.
PromoterExpressionLevel:
ThisplasmidcontainsthemammalianCAGpromoterwhichisasyntheticcompositeoftheCMVimmediateearlyenhancerfollowedbytheCBApromoterandtherabbitbetaglobinintron.TheChickenbetaactincontainsaCpGislandthatcanhelptokeepthepromoteractiveforlongerinstableculturewhencomparedtotheCMVpromoter.
SequenceandMap:
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
