PlasmidInfo:
PlasmidInformation
ProductName:pSF-Ad5-WT
ProductCode:OG617
Size(bp):38870bp
BacterialAntibioticSelection:
OriginandCompatibility:
BacterialCopyNumber:
Promoter:AdenovirusSEROtype5E1APromoter
Plasmidpurpose
Thisisaserotype5adenovirusvirusplasmidvectorthatallowsfortheproductionofreplicatingwild-typeadenoviruses.Thevectorcontainsthecompleteadenovirusserotype5genomeclonedintoa plasmidbackbonewiththekanamycinantibioticselectionthatallowsforthepropagation ofthevectorinE.coli.
RecoveryandGrowth:
VirusrecoveryrequirestwostepsfirstlytheplasmidmustbecleavedwiththePacIrestrictionenzymetolinearisethevirusgenome.Thenthelineargenomecanberecoveredandgrowninmammalian.Atypical recoverytransfectionwoulduseapproximately5ugofDNA.ItisnotnecessarytoremovetheplasmidbackboneafterlinearisingtheDNAastandardDNAcleanupwillbesufficientratherthangelextraction.Viruscoloniesshouldappearapproximately10-15daysaftertransfectioninto293cellshoweverclonescansometimesappearatearliertimepoints.Cellshouldbeculturedin2%fetalcalfserumtopreventovergrowthduringthistime
SequenceandMap:
OtherInfo:
OtherInformationCloning:
CloningIPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastProplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
