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Oxford Genetics/pBR322 (OG589) pBR322 Low Copy Cloning Vector/OG589/1 Ea
PlasmidInfo:
PlasmidInformation
ProductName:pBR322
ProductCode:OG589
Size(bp):4361bp
BacterialAntibioticSelection:
OriginandCompatibility:
BacterialCopyNumber:
Promoter:
PlasmidPurpose:
ThisplasmidcontainstheBR322(lowcopynumber)originofreplicationandprovidesapproximately20plasmidcopiesperbacterialcell.ItalsocontainsthemammalianCMVpromoterupstreamoftheMCSandKanresistanceforselectionoftransfectedmammaliancells.Lowcopynumberplasmidsareusefulforverylargetransgenesorfortransgenesthatcanbetoxictobacterialcells.
SequenceandMap:
OtherInfo:
TranscriptionTermination:Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.
Cloning:
BR322Information:BR322isawildtypeplasmidisolatedfromE.coli.Itcontainsalowcopyoriginthatallowsforplasmidmaintenanceatafrequencyofapproximately10-100plasmidcopiespercell.Typicallythisnumberiscloserto30-40copiespercell.MostcommoncloningplasmidscontainaderivativeoftheoriginofreplicationinthisplasmidcalledpUC.ThepUCoriginwascreatedbyremovingtheReprepressorproteinthatnormallyregulatesplasmidcopynumberinE.coliandalsomakingasinglepointmutationintheoriginofreplicationitself.Thepointmutationisthemostimportantdifferencebetweentheoriginofreplication.
Thisplasmidcanbeusedasageneralpurposelowcopycloningvector.
IPStatus:
IntellectualPropertyStatusThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere
